Neymar, 29, has been sidelined since picking up the injury in the French Cup win over second division side Caen on Feb. 10.
The Brazil international returned to light training a few days ago but PSG confirmed on Tuesday that this week’s second leg against Barca in the round-of-16 of the Champions League comes too soon.
“He’s very sad,” PSG coach Mauricio Pochettino said when Neymar was denied the chance to play against his former side last month.
“He was really looking forward to going to Barcelona. He loves Barcelona. He’s a player who loves to play these games, he’s made for them.”
Sources have told ESPN that Pochettino considered adding him to the squad but didn’t want to take any risks and Neymar agreed with the decision.
However, he was fit for the latter stages of last year’s competition, helping PSG reach the final, where they were beaten 1-0 by Bayern Munich.
Information from ESPN’s Julien Laurens has been included in this report.